Overview

  • Product name

    Rabbit F(ab) Anti-Pig IgG H&L
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab) polyclonal Secondary Antibody to Pig IgG - H&L
  • Host species

    Rabbit
  • Target species

    Pig
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICCmore details
  • Immunogen

    Highly purified whole immunoglobulin from pig

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Fab and F(ab')2 are respectively mono and bivalent fragments of immunoglobulins, i.e. including one or two sites for the fixation of the antigen. They are prepared by enzymic digestion (papain or pepsin). They are purified by affinity chromatography and are thus particularly specific.
    Fab and F(ab')2 fragments have certain advantages with respect to the entire antibodies in immunohistochemistry, immunocytology and flow cytometry tests.

    On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig’s are.

    On the other hand their use also increase the sensitivity of the technique. In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (this is so particularly for Fab).

    Finally, conjugate Fab\'s have a label/antigenic ratio that is twice as much compared to antibodies (except fluorescent conjugates).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

References

ab136727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab136727.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up