Rabbit F(ab) Anti-Pig IgG H&L (HRP) (ab136735)
Key features and details
- Rabbit F(ab) Anti-Pig IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Overview
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Product name
Rabbit F(ab) Anti-Pig IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Pig -
Tested applications
Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P, WBmore details -
Immunogen
Highly purified whole immunoglobulin from pig.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 49% Tris buffered saline, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Preparation of Substrate Solutions:
ELISA: OPD (orthophenylene diamine): 0.4 mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4 µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
Immunocytochemistry: DAB (diamino-benzidine): 0.5 mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5 µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
Western Blot: 4CN (4-chloro-1naphtol): 25 mg in 49.5 ml of 0.1M pH 7.4 PBS + 5 µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 minutes at room temperature. -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/500 - 1/5000.
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ICC/IF |
1/20 - 1/100.
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IHC-Fr |
1/20 - 1/100.
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IHC-P |
1/20 - 1/100.
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WB |
1/100 - 1/1000.
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Notes |
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ELISA
1/500 - 1/5000. |
ICC/IF
1/20 - 1/100. |
IHC-Fr
1/20 - 1/100. |
IHC-P
1/20 - 1/100. |
WB
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab136735 has been referenced in 2 publications.
- Chapagain S et al. Japanese encephalitis virus persists in the human reproductive epithelium and porcine reproductive tissues. PLoS Negl Trop Dis 16:e0010656 (2022). PubMed: 35905074
- Udenze D et al. CpG content in the Zika virus genome affects infection phenotypes in the adult brain and fetal lymph nodes. Front Immunol 13:943481 (2022). PubMed: 35983032