Overview

  • Product name

    Rabbit F(ab) Anti-Pig IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab) polyclonal Secondary Antibody to Pig IgG - H&L (HRP)
  • Host species

    Rabbit
  • Target species

    Pig
  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P, WBmore details
  • Immunogen

    Highly purified whole immunoglobulin from pig.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 49% Tris buffered saline, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Preparation of Substrate Solutions:

    ELISA: OPD (orthophenylene diamine): 0.4 mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4 µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm

    Immunocytochemistry: DAB (diamino-benzidine): 0.5 mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5 µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.

    Western Blot: 4CN (4-chloro-1naphtol): 25 mg in 49.5 ml of 0.1M pH 7.4 PBS + 5 µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 minutes at room temperature.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/5000.
ICC/IF 1/20 - 1/100.
IHC-Fr 1/20 - 1/100.
IHC-P 1/20 - 1/100.
WB 1/100 - 1/1000.

References

ab136735 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab136735.
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