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    rabbit-fab-sheep-igg-hl-ab136726.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit F(ab) Anti-Sheep IgG H&L (ab136726)

  • Datasheet
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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit F(ab) Anti-Sheep IgG H&L
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICC

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Overview

  • Product name

    Rabbit F(ab) Anti-Sheep IgG H&L
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Sheep
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICCmore details
  • Immunogen

    Highly purified whole immunoglobulin from sheep

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Fab and F(ab')2 are respectively mono and bivalent fragments of immunoglobulins, i.e. including one or two sites for the fixation of the antigen. They are prepared by enzymic digestion (papain or pepsin). They are purified by affinity chromatography and are thus particularly specific.
    Fab and F(ab')2 fragments have certain advantages with respect to the entire antibodies in immunohistochemistry, immunocytology and flow cytometry tests.
    On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig’s are.
    On the other hand their use also increase the sensitivity of the technique. In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (this is so particularly for Fab).
    Finally, conjugate Fab's have a label/antigenic ratio that is twice as much compared to antibodies (except fluorescent conjugates).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Sheep
    • IgG
    • Unconjugated

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab136726 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr Use at an assay dependent concentration.
    IHC-P Use at an assay dependent concentration.
    Flow Cyt Use at an assay dependent concentration.
    ICC Use at an assay dependent concentration.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab136726? Please let us know so that we can cite the reference in this datasheet.

    ab136726 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Blocking of unspecific staining of an anti-Goat/HRP secondary antibody on sheep tissue

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    In our application, we wanted to use this product to block the nonspecific staining found with a Rb anti-Goat/HRP antibody on sheep tissue. (Formalin fixed, Paraffin embedded)
    It did reduce the Background but could not fully block the unspecific binding. Therefore it could not be used for this application.

    Abcam user community

    Verified customer

    Submitted Oct 13 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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