Key features and details
- Rabbit F(ab')2 Anti-Chicken IgY H&L
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, ICC, IHC-P
Product nameRabbit F(ab')2 Anti-Chicken IgY H&L
See all IgY secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgY and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: WB, ELISA, ICC, IHC-Pmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Our Abpromise guarantee covers the use of ab102332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000.|
|ELISA||1/100 - 1/30000. Coating: 1/100 - 1/500. Primary: 1/1000 - 1/30000.|
|ICC||1/200 - 1/2000.|
|IHC-P||1/200 - 1/2000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102332 has not yet been referenced specifically in any publications.