Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase) (ab136764)

Overview

  • Product name

    Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab')2 polyclonal Secondary Antibody to Dog IgG - H&L (AP)
  • Host species

    Rabbit
  • Target species

    Dog
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, Functional Studies, ELISA, WB, ICCmore details
  • Immunogen

    Highly purified whole immunoglobulin from dog

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 48.9% Tris buffered saline, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136764 is purified by affinity chromatography and is thus particularly specific.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Preparation of Substrate Solutions:

    ELISA : 1M diethanolamin, 0.5mM MgCl2, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V/5 K2HP04 2M. Read at 405 nm.
    Immunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS/HCI, 0.5M NaCl, 0.05M MgCl2, pH 9.5).
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/25 - 1/100.
IHC-P 1/25 - 1/100.
Functional Studies Use at an assay dependent concentration.
ELISA 1/500 - 1/5000.
WB 1/100 - 1/1000.
ICC 1/25 - 1/100.

References

ab136764 has not yet been referenced specifically in any publications.

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