Overview

  • Product name

    Rabbit F(ab')2 Anti-Dog IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab')2 polyclonal Secondary Antibody to Dog IgG - H&L (HRP)
  • Host species

    Rabbit
  • Target species

    Dog
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICC, WB, ELISAmore details
  • Immunogen

    Highly purified whole immunoglobulin from dog

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 50% Tris buffered saline, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136759 is purified by affinity chromatography and is thus particularly specific.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Preparation of Substrate Solutions:

    - ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
    - Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
    - Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20 - 1/100.
IHC-P 1/20 - 1/100.
Flow Cyt Use at an assay dependent concentration.
ICC 1/20 - 1/100.
WB 1/100 - 1/1000.
ELISA 1/500 - 1/5000.

References

This product has been referenced in:

  • Vogt CM  et al. Oral Application of Recombinant Bacillus subtilis Spores to Dogs Results in a Humoral Response against Specific Echinococcus granulosus Paramyosin and Tropomyosin Antigens. Infect Immun 86:N/A (2018). Read more (PubMed: 29229735) »
See 1 Publication for this product

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