Key features and details
- Rabbit F(ab')2 Anti-Goat IgG - H&L (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Product nameRabbit F(ab')2 Anti-Goat IgG - H&L (AP), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Goat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.001% Zinc chloride, 0.01% Magnesium chloride, 0.788% Tris HCl, 0.876% Sodium chloride, 1% BSA, 50% Glycerol (glycerin, glycerine)
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab5756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/2000.|
|Dot blot||1/5000 - 1/50000.|
|WB||1/500 - 1/5000.|
|ELISA||1/10000 - 1/40000.|
|IHC||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5756 has not yet been referenced specifically in any publications.