Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed (ab102348)

Overview

  • Product name

    Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
  • Host species

    Rabbit
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-P, ICC, ELISA, WBmore details
  • Minimal
    cross-reactivity


    Human, Mouse, Rat more details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000.
ICC 1/200 - 1/1000.
ELISA 1/10000 - 1/200000. (Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

References

ab102348 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Secondary Ab: Biotinylated rabbit anti-Goat F(ab’)2 (Abcam ab102348) Concentration 1:500 30’ RT
Labeling system : Avidin-biotin complex (Vector Lab, Vectastain Elite ABC kit PK-6100) 30’ RT

Abcam user community

Verified customer

Submitted Jul 24 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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