Key features and details
- Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cyt
Product nameRabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Tested applicationsSuitable for: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cytmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Goat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Our Abpromise guarantee covers the use of ab5753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|ICC/IF||1/1000 - 1/5000.|
|ELISA||1/20000 - 1/100000.|
|IHC||1/500 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|Flow Cyt||1/200 - 1/1000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5753 has been referenced in 1 publication.
- Chamberlain G et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt . PubMed: 18698345