Overview

  • Product name

    Rabbit F(ab')2 Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Goat
  • Specificity

    This antibody reacts with Goat heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-pepsin.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Immunogen

    Full length (Goat) IgG whole molecules.

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    4.1 moles FITC per mole of Rabbit IgG F(ab')2 Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm; Emission Wavelength: 528 nm.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes


    This antibody is suitable for antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000 - 1/10000.
ICC/IF 1/500 - 1/2500.

References

ab47846 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab47846.
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