Key features and details
- Rabbit F(ab')2 Anti-Goat IgG H&L (PE)
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA (IgG and protease free)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by pepsin digesion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.
Conjugation notesConjugated to R-Phycoerythrin (R-PE) (MW 240kD) from seaweed. Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm
Our Abpromise guarantee covers the use of ab59588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab59588 has been referenced in 1 publication.
- Itoh H et al. Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells. J Vet Med 2017:5701016 (2017). PubMed: 28900631