Rabbit F(ab')2 Anti-Goat IgG H&L (PE) (ab59588)
Key features and details
- Rabbit F(ab')2 Anti-Goat IgG H&L (PE)
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF
Overview
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Product name
Rabbit F(ab')2 Anti-Goat IgG H&L (PE)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Immunogen
Full length protein corresponding to Goat IgG.
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA (IgG and protease free) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by pepsin digesion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. -
Conjugation notes
Conjugated to R-Phycoerythrin (R-PE) (MW 240kD) from seaweed. Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt
1/100 - 1/250. |
ICC/IF
1/100 - 1/250. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab59588 has been referenced in 1 publication.
- Itoh H et al. Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells. J Vet Med 2017:5701016 (2017). PubMed: 28900631