Product nameRabbit F(ab')2 Anti-Goat IgG H&L (Texas Red ®) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Goat IgG whole molecule
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 1.45% Sodium chloride, 1% BSA, 0.3% Potassium phosphate
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.6 moles Texas Redä per mole of Rabbit IgG F(ab’)2
Our Abpromise guarantee covers the use of ab5751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|Flow Cyt||1/500 - 1/2500.|
|ELISA||1/10000 - 1/50000.|
ab5751 has not yet been referenced specifically in any publications.