Rabbit F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab98780)

Overview

  • Product name

    Rabbit F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
  • Host species

    Rabbit
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Minimal
    cross-reactivity


    Human, Rat more details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary ELISA).
WB 1/5000 - 1/50000. (Colourimetric: 1/5000-1/30000, Chemiluminescent: 1/10000-1/50000).
ICC Use at an assay dependent dilution.

References

ab98780 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.
As this antibody has only been purified against it immunogen, it may cross- react with other species.
I have picked out three pre-adsorbed secondary antibodies, which show minimal cross-reactivity to human IgG:
https://www.abcam.com/index.html?datasheet=7075 https://www.abcam.com/index.html?datasheet=7075.
https://www.abcam.com/index.html?datasheet=98780 https://www.abcam.com/index.html?datasheet=98780.
https://www.abcam.com/index.html?datasheet=98808 https://www.abcam.com/index.html?datasheet=98808.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up