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  1. Link

    rabbit-fab2-mouse-igg-hl-pe-pre-adsorbed-ab7000.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)

  • Datasheet
  • SDS
Submit a review Submit a question References (5)

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Promotion Information

Abpromise

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Key features and details

  • Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Rabbit
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr

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Overview

  • Product name

    Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity


    Human
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality

    Polyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Phycoerythrin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Notes
ICC/IF
1/100 - 1/250.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab7000? Please let us know so that we can cite the reference in this datasheet.

ab7000 has been referenced in 5 publications.

  • Tian L  et al. IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application. Mol Ther Oncolytics 20:187-198 (2021). PubMed: 33665357
  • Shi C  et al. NEAT1 promotes the repair of abdominal aortic aneurysms of endothelial progenitor cells via regulating miR-204-5p/Ang-1. Am J Transl Res 13:2111-2126 (2021). PubMed: 34017378
  • Amjadi F  et al. Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women. Reprod Biomed Online N/A:N/A (2018). WB, IHC-P . PubMed: 29729850
  • Lin Y  et al. Isoliquiritigenin inhibits the proliferation and induces the differentiation of human glioma stem cells. Oncol Rep 39:687-694 (2018). PubMed: 29251326
  • Liu XL  et al. Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural-like cells. Mol Med Rep 17:7902-7910 (2018). PubMed: 29620288

Customer reviews and Q&As

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