Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)

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Overview

  • Product name
    Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality
    Polyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.

References

This product has been referenced in:
  • Amjadi F  et al. Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women. Reprod Biomed Online N/A:N/A (2018). WB, IHC-P . Read more (PubMed: 29729850) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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