Overview

  • Product name

    Rabbit F(ab')2 Anti-Mouse IgG - H&L (TR), pre-adsorbed
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Human more details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.3 moles Texas Red per mole of Rabbit IgG F(ab’)2
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:

See 1 Publication for this product

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