Key features and details
- Rabbit F(ab')2 Anti-Rat IgG H&L
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Product nameRabbit F(ab')2 Anti-Rat IgG H&L
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Our Abpromise guarantee covers the use of ab102200 in the following tested applications.
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).|
|WB||1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent 1/1000 - 1/30000.|
|ICC||1/200 - 1/2000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102200 has not yet been referenced specifically in any publications.