Overview

  • Product name

    Rabbit F(ab')2 Anti-Rat IgG - H&L (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Description

    Rabbit F(ab')2 Anti-Rat IgG - H&L (HRP), pre-adsorbed
  • Host species

    Rabbit
  • Target species

    Rat
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG, whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
WB
ELISA
Electron Microscopy
IHC
  • Application notes
    Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/3000 - 1/12000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/3000 to 1/12000 of the reconstitution concentration is suggested for this product.

    Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab6250 has not yet been referenced specifically in any publications.

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