Product nameRabbit F(ab')2 Anti-Sheep IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
DescriptionRabbit F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (AP)
Tested applicationsSuitable for: IHC-Fr, IHC-P, Flow Cyt, ICC, WB, ELISAmore details
Highly purified whole immunoglobulin from Sheep.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 48.9% Tris buffered saline, 50% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab136765 is purified by affinity chromatography and is thus particularly specific.
General notesPreparation of Substrate Solutions:
ELISA : 1M diethanolamin, 0.5mM MgCl2, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V/5 K2HP04 2M. Read at 405 nm.
Immunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS/HCI, 0.5M NaCl, 0.05M MgCl2, pH 9.5).
Our Abpromise guarantee covers the use of ab136765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/25 - 1/100.|
|IHC-P||1/25 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration.|
|ICC||1/25 - 1/100.|
|WB||1/100 - 1/1000.|
|ELISA||1/500 - 1/5000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab136765 has not yet been referenced specifically in any publications.