By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse, rat IgG was detected. This antibody may cross react with IgG from other species.
Antiserum was solid phase absorbed to ensure class specificity. Antiserum was cross absorbed using human, mouse, rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Fragments were conjugated to FITC.