Key features and details
- Rabbit polyclonal Secondary Antibody to Goat IgG - Fc (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameRabbit Anti-Goat IgG Fc (HRP) preadsorbed
See all IgG secondary antibodies
DescriptionRabbit polyclonal Secondary Antibody to Goat IgG - Fc (HRP), pre-adsorbed
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Human, Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 0.05% CMIT/MIT based preservative
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab102345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/200 - 1/5000.|
|IHC-P||1/200 - 1/5000.|
|ELISA||1/10000 - 1/100000. (Primary).|
|WB||1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102345 has not yet been referenced specifically in any publications.