Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097)

Overview

  • Product name

    Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.10
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notes

    Molar enzyme: antibody protein ratio is 1:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. (Primary).
WB 1/5000 - 1/50000. (Chemiluminescent).

Images

  • All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) at 1/5000 dilution
    Lanes 3-4 : Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) at 1/20000 dilution
    Lanes 5-6 : Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 minutes

References

ab97097 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
https://www.abcam.com/index.html?datasheet=29889 https://www.abcam.com/index.html?datasheet=29889.
MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
https://www.abcam.com/index.html?datasheet=30304 https://www.abcam.com/index.html?datasheet=30304.
With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
https://www.abcam.com/index.html?datasheet=29745 https://www.abcam.com/index.html?datasheet=29745.
I hope this information is helpful and wish you good luck with your research.

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