Product nameRabbit Anti-Goat IgG H&L (Biotin)
See all IgG secondary antibodies
Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
Goat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Our Abpromise guarantee covers the use of ab6740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC-P: 1/100 (PMID 18930986).
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This product has been referenced in:
- Costa GMJ et al. Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides). Gen Comp Endocrinol N/A:N/A (2018). Read more (PubMed: 29966660) »
- Cockrell AS et al. A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques. Sci Rep 8:10727 (2018). Read more (PubMed: 30013082) »