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I have orderd Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (Biotin) (ab97098) for Anti-Hepatitis C Virus genotype 1a NS5 antibody (ab20773). In datasheet of ab97098, it suggests to use in ELISA :1/20000 - 1/400000.((Primary).). 1/20000 is ok for the dilution or I have to have a test of it? Which concentration is fine? THank you so much.
Asked on Nov 18 2011
Thank you for contacting us. The recommended dilution appeared on the datasheet usually works in the corresponding application. However, optimization of antibody concentration may be required in individual experiment. Sometimes, you may need antibody concentration higher/lower than the recommended concentration range. For the first trial, I would suggest you to try 1/20000 first to see how is the result of ELISA. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 18 2011