Key features and details
- Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Fr
Product nameRabbit Anti-Goat IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Frmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Goat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Our Abpromise guarantee covers the use of ab7131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Dot blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|WB||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution. PubMed: 20838433|
|IHC-Fr||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7131 has been referenced in 1 publication.
- Fernandez P et al. Strain-transcendent immune response to recombinant Var2CSA DBL5-e domain block P. falciparum adhesion to placenta-derived BeWo cells under flow conditions. PLoS One 5:e12558 (2010). Flow Cyt . PubMed: 20838433