Rabbit Anti-Goat IgG H&L (DyLight® 550) preadsorbed (ab96916)

Overview

  • Product name

    Rabbit Anti-Goat IgG H&L (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Description

    Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 550), pre-adsorbed
  • Host species

    Rabbit
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab96916 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for the details provided. I am sorry you are having difficulty with these products.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab27985 and ab96916.

1) Increase the Triton X to 0.25% for 10 min to ensure permeabilization so the nuclear stain can be seen fully

2) Add a no primary control to see if the background is from the secondary. If it is, you may wish to use 10% rabbit serum instead of 5% BSA to block background from the secondary.

3) Try increasing primary antibody incubation to ON at 4C to ensure specific signal is strong.

4) Add extra washes after primary and secondary to reduce background.


Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up