Overview

  • Product name

    Rabbit Anti-Goat IgG H&L (DyLight® 650)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Conjugation

    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab96930 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

I understand your concern. The antibody has not been tested in western blotting because it is assumed that people will use fluorophore-conjugated secondaries for immunocytochemistry or histochemistry (ICC and IHC). Using fluorescent secondaries for western blotting is relatively rare. However, if you have a fluorescence imager and have had success imaging fluorescent western blots with ab28445, then ab96930 will work for you, assuming the imager can excite and read emission at the specified wavelengths. It is simply binding to a primary goat antibody, and it will be as capable of binding goat IgG on blots as it is of binding goat IgG on cells (ICC) or tissues (IHC). If you still have concerns, I will be happy to discuss other options. We do not, however, have any other fluorescent anti-goat IgG secondaries that have been tested for western blotting.

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