Product nameRabbit Anti-Goat IgG H&L (Glucose Oxidase)
See all IgG secondary antibodies
Tested applicationsSuitable for: IHC-P, IHC-Fr, ELISA, ICC, WBmore details
Highly purified whole immunoglobulin from Goat.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab136719 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
General notesDo not store in plastic tubes (i.e. eppendorf) to avoid losing activity.
Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
Our Abpromise guarantee covers the use of ab136719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/100.|
|IHC-Fr||1/10 - 1/100.|
|ELISA||1/100 - 1/1000.|
|ICC||1/10 - 1/100.|
ab136719 has not yet been referenced specifically in any publications.