Key features and details
- Rabbit Anti-Goat IgG H&L (Glucose Oxidase)
- Conjugation: Glucose Oxidase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WB
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab136719 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
General notesDo not store in plastic tubes (i.e. eppendorf) to avoid losing activity.
Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
Our Abpromise guarantee covers the use of ab136719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/100.|
|IHC-Fr||1/10 - 1/100.|
|ELISA||1/100 - 1/1000.|
|ICC||1/10 - 1/100.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab136719 has not yet been referenced specifically in any publications.