Rabbit Anti-Goat IgG H&L (Glucose Oxidase) (ab136719)
- Datasheet
- References
- Protocols
Overview
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Product name
Rabbit Anti-Goat IgG H&L (Glucose Oxidase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Tested applications
Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WBmore details -
Immunogen
Highly purified whole immunoglobulin from Goat.
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Conjugation
Glucose Oxidase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136719 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab136719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| IHC-Fr | 1/10 - 1/100. | |
| ELISA | 1/100 - 1/1000. | |
| ICC | 1/10 - 1/100. | |
| WB | 1/400. |
Datasheets and documents
References
ab136719 has not yet been referenced specifically in any publications.
