Rabbit Anti-Goat IgG H&L (Glucose Oxidase) (ab136719)
Key features and details
- Rabbit Anti-Goat IgG H&L (Glucose Oxidase)
- Conjugation: Glucose Oxidase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WB
Overview
-
Product name
Rabbit Anti-Goat IgG H&L (Glucose Oxidase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Tested applications
Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WBmore details -
Immunogen
Highly purified whole immunoglobulin from Goat.
-
Conjugation
Glucose Oxidase
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136719 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
-
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab136719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| IHC-Fr | 1/10 - 1/100. | |
| ELISA | 1/100 - 1/1000. | |
| ICC | 1/10 - 1/100. | |
| WB | 1/400. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab136719 has not yet been referenced specifically in any publications.
