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    rabbit-goat-igg-hl-glucose-oxidase-ab136719.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Goat IgG H&L (Glucose Oxidase) (ab136719)

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Key features and details

  • Rabbit Anti-Goat IgG H&L (Glucose Oxidase)
  • Conjugation: Glucose Oxidase
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WB

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Overview

  • Product name

    Rabbit Anti-Goat IgG H&L (Glucose Oxidase)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Goat
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WBmore details
  • Immunogen

    Highly purified whole immunoglobulin from Goat.

  • Conjugation

    Glucose Oxidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136719 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.

    Preparation of Substrate Solutions:

    ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.

    Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Enzyme
    • Glucose Oxidase

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab136719 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/10 - 1/100.
    IHC-Fr 1/10 - 1/100.
    ELISA 1/100 - 1/1000.
    ICC 1/10 - 1/100.
    WB 1/400.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab136719? Please let us know so that we can cite the reference in this datasheet.

    ab136719 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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