Overview

  • Product name
    Rabbit Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Goat
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    Goat IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/50000 - 1/100000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
WB 1/5000 - 1/20000. PubMed: 17369188

Images

  • Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
    Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
    Ab6741 was used at dilution 1/5000 with the primary antibody ab23938 in WB. See the review on ab23938.
  • ELISA - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
    ELISA - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
    Ab6741 was used at dilution 1/1000 with the primary antibody ab2247 in ELISA. See the review on ab2247.

References

This product has been referenced in:
  • Martin LJ  et al. Electric fields control the orientation of peptides irreversibly immobilized on radical-functionalized surfaces. Nat Commun 9:357 (2018). Read more (PubMed: 29367659) »
  • Richter J  et al. CK1a overexpression correlates with poor survival in colorectal cancer. BMC Cancer 18:140 (2018). WB . Read more (PubMed: 29409464) »

See all 71 Publications for this product

Publishing research using ab6741? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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20 reviews or Q&A

10
Votes: Highest First

DO you know the expirate date of conjugated secondary antibodies? The secondary antibodies are same that primaries (4°C 1-2 Weeks and then to -20°C) https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Goat-IgG-HL-HRP-ab6741.html

We have recently concluded a stability study for conjugated antibodies. Based on the results of this study we have decided to issue the following guidelines: Biotin conjugates +4°C short term. Aliquot and store at -20°C. Avoid freeze/thaw...

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I have purchased the following antibodies from you. Could you please send me Material Safety Data Sheet (MSDS) for these items?
1. Anti-beta Actin antibody (Cat: ab8229)
2. Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (Cat: ...

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Thank you for contacting us.

Here are the links for the MSDS for the 2 products:

For ab6741: https://www.abcam.com/ps/Products/6/ab6741/Msds/ab6741_WPNA_EN.pdf
For ab8229: https://www.abcam.com/ps/Products/8/ab8229/Msds/ab8229_WPN...

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What is best secondary antibody you can offer for ab10256.

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. ...

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Our customer purchased 1 vial of ab6741 and 1 vial of ab6721 last December when we had some promotions.
He stored it 4C and openend it this morning to test.
But there were severe precipitation in the vial (ab6721, Lot.GR27131-6, ON.1005934). ...

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Thank you for your email. I am sorry to hear that you are experiencing problems with these antibodies.

The brown precipitates with HRP conjugated antibodies are very common. We recommend customers to pipette up and down and vortexing the vial...

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Thanks for your reply. I did get some non-specific binding with the secondary only control but not as high as with both primary and secondary. I am using a very sensitive ECL reagent that we are developing and I believe I get the background ...

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Thank you for your reply.

I was wondering if ab8227 would be a good replacement for you:

https://www.abcam.com/beta-Actin-antibody-Loading-Control-ab8227.html



If it is, then please let me know and I will get it ...

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I recieved ab7125 as a replacement for ab6741 and tried it. I still have the same problem of non-specific background binding. I still believe as I originally thought that the primary antibody ab8229 may not be working. I have run controls wh...

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Thank you for your reply.

I am sorry the replacement secondary did not solve this problem. Do you get the same non-specific binding pattern when you do your secondary only control? I ask, because if you do, then that does indicate that the is...

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I would be happy to try the replacement.

Thanks.

Thank you for your reply.

As ab6741 did not prove to be successful for you, I am sending you ab7125 as a free of charge replacement. Your ne order number is ******* and you should have the new product by 10.30am tomorrow morning.

If...

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Regarding product ab6741, I did change the blocking reagent to BSA with no success. There was still high background. I also tried using blocking agent at every step, i.e. I put the blocking agent in with the secondary antibody. In that case I got no si...

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Thank you for your reply.

I am sorry that you are continuing to have problems with ab6741 in western blot. Therefore I would like to send you ab7125 as a free of charge replacement:

https://www.abcam.com/Donkey-polyclonal-Secondary-...

Read More

High background from the secondary antibody.

Thank you for contacting Abcam earlier today.

If you are still experiencing any issues with ab6741 in western blot after trying the protocol tips that we discussed, please let me know and I will be happy to look into sending a new antibody to...

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I'm confused because when I had inquiry about the precipitation before, I received emailusing them with gentle vortex but,
this time, you answered spin down it and use only supernatant.
Let me know which method is right.


Best...

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Thank you for your reply.

I can confirm that for the HRP conjugated antibodies the approach that Irecommended is the correct one. Spining the vial down will prevent free HRP to bind to un-specifically to samples and therefore avoid increased ...

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1-10 of 20 Abreviews or Q&A

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