Overview

  • Product name
    Rabbit Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000.
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Images

  • IHC image of beta Actin staining in Human normal Colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97100, 1/100 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes :
    Anti-beta Actin antibody (ab8229) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane. 
    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/10000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References

This product has been referenced in:
  • Okur E & Yerlikaya A A novel and effective inhibitor combination involving bortezomib and OTSSP167 for breast cancer cells in light of label-free proteomic analysis. Cell Biol Toxicol N/A:N/A (2018). Read more (PubMed: 29948483) »
  • Mennitti LV  et al. Early exposure to distinct sources of lipids affects differently the development and hepatic inflammatory profiles of 21-day-old rat offspring. J Inflamm Res 11:11-24 (2018). Read more (PubMed: 29403301) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for trying our suggestion of using ab97100 instead of the staining kit. You may want to try increasing the dilution to 1:200 to get stronger staining.

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Answer

Thank you for contacting us.

ab97100; one freeze thaw cycle should not be having any effect on quality so please recommend customer using the antibody as they would like to.

ab6721; please centrifuge out the brown precipitates. You may however need to use the high concentration of antibody than usual but the antibody will be fine. Please let us know if this antibody fails, we will then try to help you with protocol troubleshooting.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab111976. I would also appreciate if you can confirm some further details:

What is the type of sample you are working with? (Tissue / cell lysate, species)
How did you store the antibody?
Did you run a "noprimary control"? did you get a black blot using the secondary only? A "no primary" control would determine if the problem comes from the primary or the secondary.
Could you please confirm you placed an order for this antibodyvia *******?



Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

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Question
Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us with your question. For the alkaline phosphatase conjugated antibody ab97107, you can use the liquid fast-red kit ab64254- https://www.abcam.com/Liquid-Fast-Red-Substrate-Kit-ab64254.html For the HRP conjugated antibody ab97100, you can use the DAB kit ab64238- https://www.abcam.com/DAB-Substrate-Kit-ab64238.html I hope this information is helpful but please let me know if you have any questions.

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