Rabbit Anti-Goat IgG H&L (HRP) preadsorbed (ab7132)

Submit a review Q&A (4)References (3)

Overview

  • Product name

    Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Goat

  • Specificity

    Pre-adsorbed against Human Serum Proteins to minimise reactivity.

  • Tested applications

    Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICCmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Goat IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.60
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/120000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
WB 1/1000 - 1/5000.
ICC 1/500 - 1/2500.

References

This product has been referenced in:

  • Moberg M  et al. Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise. Am J Physiol Cell Physiol 310:C874-84 (2016). Read more (PubMed: 27053525) »
  • Apró W  et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). Read more (PubMed: 25605643) »
See all 3 Publications for this product

Publishing research using ab7132? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

What is best secondary antibody you can offer for ab10256.

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Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Sir/Madam,



I am trying to order an antibody from your website but i can’t seem to find the product anywhere on-line, perhaps it has been discontinued or is out of stock?



The product # is 92281 a MAFbx primary antibody. I also needed the appropriate secondary goat antibody (# 7132) which is also unavailable. Can you suggest the next best possible alternatives please? Or let me know if these antibodies are likely to be in stock sometime soon?



Many thanks

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Answer

Thank you for your enquiry and your interest in our products.



I can confirm that ab92281 is Anti-Fbx32 antibody and ab7132 is Rabbit anti-Goat IgG H&L (HRP) secondary antibody; and both products are still published and available.



ab92281: https://www.abcam.com/fbx32-antibody-ab92281.html

ab7132: https://www.abcam.com/rabbit-polyclonal-secondary-antibody-to-goat-igg-h-l-hrp-pre-adsorbed-ab7132.html



If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hi,   What secondary HRP-linked antibody would you recommend after incubation with ab92281 (FBX32 ab)?

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Answer

Thank you for your enquiry. I can recommend the following HRP-conjugated secondary antibody based on your requirements: Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab7132) I hope this is helpful. Please contact us again if you have any further questions.

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Question

I have sheep polyclonal to 5-methyl cytosine antibody (ab1884) as first antibody, and Rabbit polyclonal to goat IgG H&L (HRP) (ab7132) as second antibody. Dose rabbit IgG to goat react with sheep IgG?

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Answer

Thank you for your enquiry. I have been in touch with the lab and determined that this antibody has NOT been preabsorbed against sheep IgG. I am sorry that I cannot be of more assistance on this occasion.

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