Rabbit Anti-Goat IgG H&L (HRP) preadsorbed (ab7132)

Submit a review Q&A (4)References (5)

Key features and details

  • Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC

Overview

  • Product name

    Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Goat

  • Specificity

    Pre-adsorbed against Human Serum Proteins to minimise reactivity.

  • Tested applications

    Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICCmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Goat IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20000 - 1/120000.
IHC-P
1/500 - 1/2500.
IHC-Fr
1/500 - 1/2500.
WB
1/1000 - 1/5000.
ICC
1/500 - 1/2500.
Notes
ELISA
1/20000 - 1/120000.
IHC-P
1/500 - 1/2500.
IHC-Fr
1/500 - 1/2500.
WB
1/1000 - 1/5000.
ICC
1/500 - 1/2500.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab7132? Please let us know so that we can cite the reference in this datasheet.

ab7132 has been referenced in 5 publications.

  • Issa AR  et al. The lysosomal membrane protein LAMP2A promotes autophagic flux and prevents SNCA-induced Parkinson disease-like symptoms in the Drosophila brain. Autophagy 14:1898-1910 (2018). PubMed: 29989488
  • Rundqvist HC  et al. Influence of nutrient ingestion on amino acid transporters and protein synthesis in human skeletal muscle after sprint exercise. J Appl Physiol (1985) 123:1501-1515 (2017). PubMed: 28860165
  • Moberg M  et al. Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise. Am J Physiol Cell Physiol 310:C874-84 (2016). PubMed: 27053525
  • Apró W  et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). PubMed: 25605643
  • Kassner N  et al. CBR1 is a predominant doxorubicin reductase in the human liver. Drug Metab Dispos : (2008). WB . PubMed: 18635746

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

What is best secondary antibody you can offer for ab10256.

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Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Sir/Madam,



I am trying to order an antibody from your website but i can’t seem to find the product anywhere on-line, perhaps it has been discontinued or is out of stock?



The product # is 92281 a MAFbx primary antibody. I also needed the appropriate secondary goat antibody (# 7132) which is also unavailable. Can you suggest the next best possible alternatives please? Or let me know if these antibodies are likely to be in stock sometime soon?



Many thanks

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Answer

Thank you for your enquiry and your interest in our products.



I can confirm that ab92281 is Anti-Fbx32 antibody and ab7132 is Rabbit anti-Goat IgG H&L (HRP) secondary antibody; and both products are still published and available.



ab92281: https://www.abcam.com/fbx32-antibody-ab92281.html

ab7132: https://www.abcam.com/rabbit-polyclonal-secondary-antibody-to-goat-igg-h-l-hrp-pre-adsorbed-ab7132.html



If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hi,   What secondary HRP-linked antibody would you recommend after incubation with ab92281 (FBX32 ab)?

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Answer

Thank you for your enquiry. I can recommend the following HRP-conjugated secondary antibody based on your requirements: Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab7132) I hope this is helpful. Please contact us again if you have any further questions.

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Question

I have sheep polyclonal to 5-methyl cytosine antibody (ab1884) as first antibody, and Rabbit polyclonal to goat IgG H&L (HRP) (ab7132) as second antibody. Dose rabbit IgG to goat react with sheep IgG?

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Answer

Thank you for your enquiry. I have been in touch with the lab and determined that this antibody has NOT been preabsorbed against sheep IgG. I am sorry that I cannot be of more assistance on this occasion.

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