Product nameRabbit Anti-Goat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies
SpecificityPre-adsorbed against Human Serum Proteins to minimise reactivity.
Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WB, ICCmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Goat IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesHorseradish Peroxidase (HRP)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab7132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/120000.|
|IHC-P||1/500 - 1/2500.|
|IHC-Fr||1/500 - 1/2500.|
|WB||1/1000 - 1/5000.|
|ICC||1/500 - 1/2500.|
This product has been referenced in:
- Moberg M et al. Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise. Am J Physiol Cell Physiol 310:C874-84 (2016). Read more (PubMed: 27053525) »
- Apró W et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). Read more (PubMed: 25605643) »