Rabbit Anti-Goat IgG H&L (HRP) preadsorbed (ab97105)
Key features and details
- Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Horse, Human, Mouse, Pig, Rat more details -
Immunogen
Other Immunogen Type corresponding to Goat IgG.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. ELISA-Primary
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WB | (1) |
1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. ELISA-Primary |
WB
1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab97105 has been referenced in 10 publications.
- Reithmair M et al. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance. Methods Mol Biol 2504:113-133 (2022). PubMed: 35467283
- Kirchner B et al. Postprandial transfer of colostral extracellular vesicles and their protein and miRNA cargo in neonatal calves. PLoS One 15:e0229606 (2020). PubMed: 32109241
- Buschmann D et al. Propofol and Sevoflurane Differentially Impact MicroRNAs in Circulating Extracellular Vesicles during Colorectal Cancer Resection: A Pilot Study. Anesthesiology 132:107-120 (2020). PubMed: 31596735
- Mussack V et al. Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research. Biomol Detect Quantif 17:100089 (2019). PubMed: 31194192
- Madan E et al. HIF-transcribed p53 chaperones HIF-1a. Nucleic Acids Res 47:10212-10234 (2019). PubMed: 31538203
- Hermann S et al. Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera. J Extracell Vesicles 8:1670935 (2019). PubMed: 31632620
- Buschmann D et al. Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing. J Extracell Vesicles 7:1481321 (2018). PubMed: 29887978
- Chen T et al. Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p. Cell Prolif N/A:e12548 (2018). PubMed: 30507050
- Cimica V et al. Novel Respiratory Syncytial Virus-Like Particle Vaccine Composed of the Postfusion and Prefusion Conformations of the F Glycoprotein. Clin Vaccine Immunol 23:451-9 (2016). PubMed: 27030590
- Ogawa S et al. Shotgun proteomic analysis of porcine colostrum and mature milk. Anim Sci J 85:440-8 (2014). PubMed: 24450292