Overview

  • Product name
    Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed
  • Host species
    Rabbit
  • Target species
    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Horse, Human, Mouse, Pig, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Goat IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. ELISA-Primary
WB 1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000

References

This product has been referenced in:
  • Buschmann D  et al. Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing. J Extracell Vesicles 7:1481321 (2018). Read more (PubMed: 29887978) »
  • Cimica V  et al. Novel Respiratory Syncytial Virus-Like Particle Vaccine Composed of the Postfusion and Prefusion Conformations of the F Glycoprotein. Clin Vaccine Immunol 23:451-9 (2016). Read more (PubMed: 27030590) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer


ab65486 is no longer is our catalog.
ab97105 (https://www.abcam.com/ab97105) is an alternative rabbit polyclonal secondary antibody to Goat IgG -H&L (HRP), pre-absorbed.
The price is €205.00for 500µg, the shipping charges to Croatia are €63.00.

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Question
Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam.   I have attached the MSDS for ab97120 and ab97105, both of which contain the preservative proclin.  A MSDS is not required for ab105466.   I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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