Rabbit Anti-Guinea pig IgG H&L (HRP) (ab6771)

Submit a review Q&A (3)References (7)

Overview

  • Product name
    Rabbit Anti-Guinea pig IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Guinea pig
  • Tested applications
    Suitable for: IHC-Fr, Immunomicroscopy, WB, ICC/IF, Dot blot, ELISA, IHC-Pmore details
  • Immunogen

    guinea pig IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Horseradish Peroxidase (HRP).
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using guinea pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.

References

This product has been referenced in:
  • Mallek A  et al. Experimental diabetes induced by streptozotocin in the desert gerbil, Gerbillus gerbillus, and the effects of short-term 20-hydroxyecdysone administration. Biomed Pharmacother 102:354-361 (2018). IHC-P ; Guinea pig . Read more (PubMed: 29571020) »
  • Diaz-Castroverde S  et al. Insulin receptor isoform A ameliorates long-term glucose intolerance in diabetic mice. Dis Model Mech 9:1271-1281 (2016). ICC/IF ; Mouse . Read more (PubMed: 27562101) »
See all 7 Publications for this product

Publishing research using ab6771? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

For this antibody, where on the IgG molecule is HRP conjugated?

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Answer

Thank you for your enquiry. The HRP molecules are conjugated to the available lysines. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Customer received vial, lot#48986, and there was brown precipitate in the vial. Tried antibody, but it did not work.

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Answer

Thank you for your phone call. I'm sending you a free of charge replacement for ab6771 (a newer batch that we have in stock). For your record it is order# 42622. It is in stock in our office in Great Britain so you should receive it by Tuesday of next week. Please let me if you have any problems with the replacement.

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Question

I would like to purchase an anti-guinea pig secondary antibody, and you have a choice of appropriate antibodies raised in different species (rabbit and goat). Which factors should I consider when choosing which species of antibody to go for?

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Answer

Either of these two secondaries will be equally suitable.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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