Specific antibodies were absorpted by incubation with Sepharose-bound Guinea Pig immunoglobulins.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized Guinea Pig immunoglobulins a minimum of 65% protein bound.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended dilution as secondary antibody in ELISA is 1:1000.
Recommended dilution as secondary antibody in dot-blotting is 1:100.
This product has been referenced in:
Haase TN et al. Growth arrest specific protein (GAS) 6: a role in the regulation of proliferation and functional capacity of the perinatal rat beta cell. Diabetologia56:763-73 (2013).
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