Key features and details
- Rabbit Anti-Horse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, Immunomicroscopy, WB, IHC-P, ELISA, Dot blot
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation notesHorseradish Peroxidase (HRP)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab6921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/2500.|
|IHC-Fr||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|WB||1/5000 - 1/20000.|
|IHC-P||Use at an assay dependent dilution.|
|ELISA||1/50000 - 1/200000.|
|Dot blot||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6921 has been referenced in 8 publications.
- Spoerry C et al. Novel IgG-Degrading Enzymes of the IgdE Protease Family Link Substrate Specificity to Host Tropism of Streptococcus Species. PLoS One 11:e0164809 (2016). PubMed: 27749921
- Scheel TK et al. Characterization of nonprimate hepacivirus and construction of a functional molecular clone. Proc Natl Acad Sci U S A 112:2192-7 (2015). PubMed: 25646476
- Endmann A et al. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One 9:e101715 (2014). WB . PubMed: 24992038
- Kong L et al. HIV infection of hepatocytes results in a modest increase in hepatitis C virus expression in vitro. PLoS One 9:e83728 (2014). PubMed: 24586227
- Qin Y et al. Improved method for rapid and efficient determination of genome replication and protein expression of clinical hepatitis B virus isolates. J Clin Microbiol 49:1226-33 (2011). PubMed: 21289153
- Qin Y et al. Hepatitis B virus genotype C isolates with wild-type core promoter sequence replicate less efficiently than genotype B isolates but possess higher virion secretion capacity. J Virol 85:10167-77 (2011). PubMed: 21775451
- Guarnieri M et al. Point mutations upstream of hepatitis B virus core gene affect DNA replication at the step of core protein expression. J Virol 80:587-95 (2006). PubMed: 16378961
- Lee SC et al. Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3. J Biol Chem 280:9567-77 (2005). PubMed: 15590643