Overview

  • Product name

    Rabbit Anti-Horse IgG+IgM+IgA H&L (HRP)
    See all IgG + IgM + IgA secondary antibodies
  • Host species

    Rabbit
  • Target species

    Horse
  • Specificity

    Antibodies were raised in rabbit to Horse IgG, IgM and IgA
  • Tested applications

    Suitable for: ELISA, Dot blotmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.50
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Specific antibodies were absorpted by incubation with Sepharose-bound Horse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized Horse immunoglobulins a minimum of 65% protein bound.
  • Conjugation notes

    The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality

    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
  • Application notes
    Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • References

    ab8533 has not yet been referenced specifically in any publications.

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