Overview

  • Product name

    Rabbit Anti-Human IgA alpha chain
    See all IgA secondary antibodies
  • Host species

    Rabbit
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000
WB 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000
ICC Use at an assay dependent dilution.

References

ab97216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Positive control MY5 and negative control LP-1 (multiple myeloma) cell lines immersion-fixed in 4% PFA, permeablized, blocked in 10% donkey serum/PBS 1 hour and incubated with 1/2000 ab97216 in 10% donkey serum/PBS overnight at 4oC. Washed 3X 5 minutes PBS and incubated with 1/2000 Alexa 488 donkey anti-rabbit F(ab2)" secondary antibody (Jackson). Washed 5X 5 minutes PBS and mounted in DAPI/Vectashield. Images acquired on Zeiss AxioImager FITC channel at 50ms exposure.

Ms. Natalie Erdmann

Verified customer

Submitted Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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