Overview

  • Product name

    Rabbit Anti-Human IgA alpha chain (Biotin)
    See all IgA secondary antibodies
  • Host species

    Rabbit
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notes

    Biotin/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (as Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

References

This product has been referenced in:

  • He X  et al. Upregulation of hyaluronan-mediated motility receptor in hepatocellular carcinoma predicts poor survival. Oncol Lett 10:3639-3646 (2015). Read more (PubMed: 26788183) »
  • Hammar O  et al. Autoantibodies and gastrointestinal symptoms in infertile women in relation to in vitro fertilization. BMC Pregnancy Childbirth 13:201 (2013). ELISA . Read more (PubMed: 24191889) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The laboratory has not tested these antibodies inparallel nor have they measured the affinity constants. WE are therefore not able to tell you which antibody would have higher affinity. I can howeverreassure you, that we do guarantee both of these antibody to work as stated on the datasheet in the similar dilution ranges.

Indeed,I think that the affinity ofboth these antibodies should be enough for good results in classical applications. The difference is the host species in which they are produced.I would therefore recommend to choose the antibodies in function of the secondary antibodies you already have (e.g. anti goat or anti rabbit) and whether any double stainings need to be performed. Double stainings should be performed with antibodies from different species in order to avoid crossstaining.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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