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I found two polyclonal anti-human IgA antibodies.
One is produced in Rabbit (ab97218) and the other is produced in Goat (ab97213).
What is the difference between them in terms of affinity?
Is there any thing that I need to consider when I select the host species of antibody?
Thank you in advance.
Asked on Mar 26 2012
Thank you for contacting us.
The laboratory has not tested these antibodies inparallel nor have they measured the affinity constants. WE are therefore not able to tell you which antibody would have higher affinity. I can howeverreassure you, that we do guarantee both of these antibody to work as stated on the datasheet in the similar dilution ranges.
Indeed,I think that the affinity ofboth these antibodies should be enough for good results in classical applications. The difference is the host species in which they are produced.I would therefore recommend to choose the antibodies in function of the secondary antibodies you already have (e.g. anti goat or anti rabbit) and whether any double stainings need to be performed. Double stainings should be performed with antibodies from different species in order to avoid crossstaining.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Mar 26 2012