Key features and details
- Rabbit Anti-Human IgA H&L
- Host species: Rabbit
- Suitable for: ELISA, Dot blot
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended dilution as secondary antibody in dot-blotting is 1:100.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8508 has not yet been referenced specifically in any publications.