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Antibodies were raised in rabbit to human IgA
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
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Immunogen affinity purified
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM.
Specific antibodies were absorpted by incubation with Sepharose-bound human IgA.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized human IgA a minimum of 65% protein bound.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended dilution as secondary antibody in ELISA is 1:1000.
Recommended dilution as secondary antibody in dot-blotting is 1:100.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"