Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)

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Key features and details

  • Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISA

Overview

  • Product name

    Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Human

  • Tested applications

    Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details

  • Immunogen

    human IgG whole molecule

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.

  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • Clonality

    Polyclonal

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
WB
ICC/IF
IHC-Fr
Dot blot
IHC-P
ELISA
  • Application notes
    suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab6760? Please let us know so that we can cite the reference in this datasheet.

    ab6760 has been referenced in 4 publications.

    • Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Sci Rep 7:15817 (2017). WB . PubMed: 29150665
    • Iversen R  et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). PubMed: 23690478
    • Kap YS  et al. Fast Progression of Recombinant Human Myelin/Oligodendrocyte Glycoprotein (MOG)-Induced Experimental Autoimmune Encephalomyelitis in Marmosets Is Associated with the Activation of MOG34-56-Specific Cytotoxic T Cells. J Immunol 180:1326-37 (2008). ELISA . PubMed: 18209026
    • Jagessar SA  et al. Autoimmunity Against Myelin Oligodendrocyte Glycoprotein Is Dispensable for the Initiation Although Essential for the Progression of Chronic Encephalomyelitis in Common Marmosets. J Neuropathol Exp Neurol 67:326-340 (2008). ELISA . PubMed: 18379435

    Customer reviews and Q&As

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    Question

    does this antibody recognize human kappa light chains? lambda? both?

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    Answer

    ab6760 is produced against polyclonal whole human IgG purified from serum, and the kappa:lambda ratio of IgG normal human serum is reported to be ~2:1. ab6760 is not tested specifically against kappa or lambda light chains, but it is expected to recognize both forms as they are normally present in human serum.

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