Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)
Key features and details
- Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details -
Immunogen
human IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy | ||
| WB | ||
| ICC/IF | ||
| IHC-Fr | ||
| Dot blot | ||
| IHC-P | ||
| ELISA |
| Notes |
|---|
Application notes
suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab6760 has been referenced in 4 publications.
- Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Sci Rep 7:15817 (2017). WB . PubMed: 29150665
- Iversen R et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). PubMed: 23690478
- Kap YS et al. Fast Progression of Recombinant Human Myelin/Oligodendrocyte Glycoprotein (MOG)-Induced Experimental Autoimmune Encephalomyelitis in Marmosets Is Associated with the Activation of MOG34-56-Specific Cytotoxic T Cells. J Immunol 180:1326-37 (2008). ELISA . PubMed: 18209026
- Jagessar SA et al. Autoimmunity Against Myelin Oligodendrocyte Glycoprotein Is Dispensable for the Initiation Although Essential for the Progression of Chronic Encephalomyelitis in Common Marmosets. J Neuropathol Exp Neurol 67:326-340 (2008). ELISA . PubMed: 18379435