Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)

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Overview

  • Product name
    Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Human
  • Tested applications
    Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
WB
ICC/IF
IHC-Fr
Dot blot
IHC-P
ELISA
  • Application notes
    suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

    • References

    This product has been referenced in:
    • Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Sci Rep 7:15817 (2017). WB . Read more (PubMed: 29150665) »
    • Iversen R  et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). Read more (PubMed: 23690478) »
    See all 4 Publications for this product

    Publishing research using ab6760? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    does this antibody recognize human kappa light chains? lambda? both?

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    Answer

    ab6760 is produced against polyclonal whole human IgG purified from serum, and the kappa:lambda ratio of IgG normal human serum is reported to be ~2:1. ab6760 is not tested specifically against kappa or lambda light chains, but it is expected to recognize both forms as they are normally present in human serum.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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