Overview

  • Product name

    Rabbit Anti-Human IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Sun Y  et al. Identification of a novel HIV-1-neutralizing antibody from a CRF07_BC-infected Chinese donor. Oncotarget 8:63047-63063 (2017). ICC/IF . Read more (PubMed: 28968970) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry
Cell were stained with humanized primary antibody then incubate with this secondary and images were taken by fluorescence microscope. Works very well.

Abcam user community

Verified customer

Submitted Oct 31 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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