Key features and details
- Rabbit Anti-Human IgG H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Product nameRabbit Anti-Human IgG H&L (DyLight® 650)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
ConjugationDyLight® 650. Ex: 654nm, Em: 673nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
General notesDyLight® 650 replaces DyLight® 649.
Our Abpromise guarantee covers the use of ab96906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500. Antigen retrieval is not essential but may optimise staining.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab96906 has not yet been referenced specifically in any publications.