Rabbit Anti-Human IgG H&L (HRP) (ab6759)

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ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

    Key features and details

    • Rabbit Anti-Human IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Rabbit
    • Isotype: IgG
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Rabbit Anti-Human IgG H&L (HRP)
      See all IgG secondary antibodies

    • Host species

      Rabbit

    • Target species

      Human

    • Tested applications

      Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details

    • Immunogen

      human IgG whole molecule

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Conjugation notes

      Horseradish Peroxidase (HRP)

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6759 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Dot blot
    Use at an assay dependent dilution.
    ELISA (1)
    1/130000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/5000 - 1/25000.
    ICC/IF
    1/1000 - 1/5000.
    Competitive ELISA
    Use at an assay dependent dilution. PubMed: 20573830
    Notes
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/130000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/5000 - 1/25000.
    ICC/IF
    1/1000 - 1/5000.
    Competitive ELISA
    Use at an assay dependent dilution. PubMed: 20573830

    Images

    • ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
      ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

      ELISA results of ab6759 tested against purified human IgG protein.

      Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (55)

    Publishing research using ab6759? Please let us know so that we can cite the reference in this datasheet.

    ab6759 has been referenced in 55 publications.

    • Fan J  et al. Oncolytic herpes simplex virus and temozolomide synergistically inhibit breast cancer cell tumorigenesis in vitro and in vivo. Oncol Lett 21:99 (2021). PubMed: 33376532
    • Zhu LJ  et al. BUB1 promotes proliferation of liver cancer cells by activating SMAD2 phosphorylation. Oncol Lett 19:3506-3512 (2020). PubMed: 32269624
    • Orlowski A  et al. Successful Transduction with AAV Vectors after Selective Depletion of Anti-AAV Antibodies by Immunoadsorption. Mol Ther Methods Clin Dev 16:192-203 (2020). PubMed: 32055647
    • Yuan YF  et al. Cyclopamine functions as a suppressor of benign prostatic hyperplasia by inhibiting epithelial and stromal cell proliferation via suppression of the Hedgehog signaling pathway. Int J Mol Med 46:311-319 (2020). PubMed: 32319534
    • Wang X  et al. Adenovirus delivery of encoded monoclonal antibody protects against different types of influenza virus infection. NPJ Vaccines 5:57 (2020). PubMed: 32665862
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Intracellular Alpha-galactosidase A/antibody-complex detection from enzyme-treated EA.hy926 cells

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Cells were seeded on 96-well plates with a density of 2x105 cell/ml and grown until confluent. After 4 hours of incubation with human recombinant α-galactosidase A (AGAL, 4.6 μg/ml) and patients’ serum (50 µl per 4.6 μg/ml AGAL), cells were washed twice with PBS and lysed with passive lysis buffer (Promega, Mannheim Germany). Lysates were centrifuged (14.000x g) for 20 minutes at 4°C and supernatants were used for ELISAs, as follows. 96 well plates were coated with a capture antibody (α-AGAL, R&D Sytems, Minneapolis, US no. AF6146, 200 ng per well) over night at 4°C. After 5 washes with 200 μl PBS/Tween 0.1% and blocking with PBS/BSA 2% for 2 hours at RT, 3-10 μl of cellular lysates were applied in 100 μl PBS overnight at 4°C. Wells were washed 3 times with PBS/Tween 0.1%. For human IgG detection shuttled into cells by AGAL, anti-hIgG HRP-coupled antibodies (Abcam no: ab6759, working concentration: 0.66 μg/ml) were applied in PBS/BSA 2% for 2 hours at RT. After 5 additional washes, 100 μl 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to each well, incubated at RT, and stopped with 100 μl 2 mol/l sulfuric acid. Absorption was measured at 450 nm and normalized for protein content and against serum from a healthy control (negativ for antibodies against AGAL).

    Figure legend: *p<0.05, **p<0.01.

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    Submitted Sep 04 2019

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