Rabbit Anti-Human IgG H&L (HRP) (ab6759)
Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details -
Immunogen
human IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent dilution.
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ELISA | (1) |
1/130000.
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IHC-P |
Use at an assay dependent dilution.
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|
IHC-Fr |
Use at an assay dependent dilution.
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|
Immunomicroscopy |
Use at an assay dependent dilution.
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|
WB |
1/5000 - 1/25000.
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|
ICC/IF |
1/1000 - 1/5000.
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|
Competitive ELISA |
Use at an assay dependent dilution. PubMed: 20573830
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Notes |
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Dot blot
Use at an assay dependent dilution. |
ELISA
1/130000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/5000 - 1/25000. |
ICC/IF
1/1000 - 1/5000. |
Competitive ELISA
Use at an assay dependent dilution. PubMed: 20573830 |
Images
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ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (75)
ab6759 has been referenced in 75 publications.
- Feng Y et al. Immunogenicity of an inactivated SARS-CoV-2 vaccine in people living with HIV-1: a non-randomized cohort study. EClinicalMedicine 43:101226 (2022). PubMed: 34901799
- Pupina N et al. Immune response to a conserved enteroviral epitope of the major capsid VP1 protein is associated with lower risk of cardiovascular disease. EBioMedicine 76:103835 (2022). PubMed: 35091341
- Jaago M et al. Antibody response to oral biofilm is a biomarker for acute coronary syndrome in periodontal disease. Commun Biol 5:205 (2022). PubMed: 35246599
- Wang C et al. ImmunoPET imaging of multiple myeloma with [68Ga]Ga-NOTA-Nb1053. Eur J Nucl Med Mol Imaging 48:2749-2760 (2021). PubMed: 33543326
- Kuwada T et al. Identification of an Anti-Integrin avß6 Autoantibody in Patients With Ulcerative Colitis. Gastroenterology 160:2383-2394.e21 (2021). PubMed: 33582126