Overview

  • Product name
    Rabbit Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Human
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/130000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/5000 - 1/25000.
ICC/IF 1/1000 - 1/5000.
Competitive ELISA Use at an assay dependent dilution. PubMed: 20573830

Images

  • ELISA results of ab6759 tested against purified human IgG protein.

    Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

References

This product has been referenced in:
  • Babaei H  et al. Increased circulation mobilization of endothelial progenitor cells in preterm infants with retinopathy of prematurity. J Cell Biochem 119:6575-6583 (2018). Read more (PubMed: 29737539) »
  • Han ZM  et al. Effect of CEACAM-1 knockdown in human colorectal cancer cells. Oncol Lett 16:1622-1626 (2018). Read more (PubMed: 30008845) »
See all 20 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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