Rabbit Anti-Human IgG H&L (HRP) (ab6759)
- Datasheet
- References (23)
- Protocols
Overview
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Product nameRabbit Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies -
Host speciesRabbit
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Target speciesHuman
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Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details
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Immunogen
human IgG whole molecule
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ConjugationHRP
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Conjugation notesHorseradish Peroxidase (HRP)
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | 1/130000. | |
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| Immunomicroscopy | Use at an assay dependent dilution. | |
| WB | 1/5000 - 1/25000. | |
| ICC/IF | 1/1000 - 1/5000. | |
| Competitive ELISA | Use at an assay dependent dilution. PubMed: 20573830 |
Images
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ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
Datasheets and documents
References
This product has been referenced in:
- Babaei H et al. Increased circulation mobilization of endothelial progenitor cells in preterm infants with retinopathy of prematurity. J Cell Biochem 119:6575-6583 (2018). Read more (PubMed: 29737539) »
- Han ZM et al. Effect of CEACAM-1 knockdown in human colorectal cancer cells. Oncol Lett 16:1622-1626 (2018). Read more (PubMed: 30008845) »
