Product nameRabbit Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies
Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details
human IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesHorseradish Peroxidase (HRP)
Our Abpromise guarantee covers the use of ab6759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|WB||1/5000 - 1/25000.|
|ICC/IF||1/1000 - 1/5000.|
|Competitive ELISA||Use at an assay dependent dilution. PubMed: 20573830|
ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
This product has been referenced in:
- Babaei H et al. Increased circulation mobilization of endothelial progenitor cells in preterm infants with retinopathy of prematurity. J Cell Biochem 119:6575-6583 (2018). Read more (PubMed: 29737539) »
- Han ZM et al. Effect of CEACAM-1 knockdown in human colorectal cancer cells. Oncol Lett 16:1622-1626 (2018). Read more (PubMed: 30008845) »