Rabbit Anti-Human IgG H&L (HRP) (ab6759)
Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details -
Immunogen
human IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent dilution.
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ELISA | (1) |
1/130000.
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IHC-P |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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|
Immunomicroscopy |
Use at an assay dependent dilution.
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|
WB |
1/5000 - 1/25000.
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ICC/IF |
1/1000 - 1/5000.
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|
Competitive ELISA |
Use at an assay dependent dilution. PubMed: 20573830
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Notes |
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Dot blot
Use at an assay dependent dilution. |
ELISA
1/130000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/5000 - 1/25000. |
ICC/IF
1/1000 - 1/5000. |
Competitive ELISA
Use at an assay dependent dilution. PubMed: 20573830 |
Images
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ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (115)
ab6759 has been referenced in 115 publications.
- Zhang X et al. Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer. Exp Ther Med 25:29 (2023). PubMed: 36561617
- Umemoto A et al. Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 25:25 (2023). PubMed: 36804906
- Zeng Q et al. TRIM59 attenuates ox-LDL-induced endothelial cell inflammation, apoptosis, and monocyte adhesion through AnxA2. Ann Transl Med 11:42 (2023). PubMed: 36819529
- Martin-Souto L et al. Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients. J Fungi (Basel) 9:N/A (2023). PubMed: 36836272
- Yang J et al. KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma. Exp Ther Med 25:154 (2023). PubMed: 36911368