Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Product nameRabbit Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
Our Abpromise guarantee covers the use of ab97160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/5000.|
|ELISA||1/10000 - 1/100000. (Primary).|
|WB||1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000|
|ICC||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97160 has been referenced in 2 publications.
- Marcandalli J et al. Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus. Cell 176:1420-1431.e17 (2019). PubMed: 30849373
- Rieder FJ et al. Microbial Cryptotopes are Prominent Targets of B-cell Immunity. Sci Rep 6:31657 (2016). ELISA . PubMed: 27539094