Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Rabbit Anti-Human IgG H&L (HRP) preadsorbed (ab7160)

Submit a review Q&A (2)

Key features and details

  • Rabbit Anti-Human IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

Overview

  • Product name

    Rabbit Anti-Human IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Human

  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details

  • Minimal
    cross-reactivity


    Mouse more details

  • Immunogen

    human IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
ELISA
1/72000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ICC/IF
1/1000 - 1/5000.
Notes
Dot blot
Use at an assay dependent concentration.
ELISA
1/72000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ICC/IF
1/1000 - 1/5000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab7160? Please let us know so that we can cite the reference in this datasheet.

ab7160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Does this antibody recognize all IgG subtypes?

Read More
Answer

Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

Read More

Question

Hello, I was wondering if any of your anti-human antibodies were tested against Fab' fragments. I wanted to make sure they would pick up heavy and light chain of a Fab' molecule. Thanks, Aparna

Read More
Answer

This antibody has been shown to react with both the heavy and light chains of human IgG.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com