Rabbit Anti-Human IgG H&L (HRP) preadsorbed (ab7160)

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Overview

  • Product name
    Rabbit Anti-Human IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Human
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity

    Mouse more details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/72000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
ICC/IF 1/1000 - 1/5000.

References

ab7160 has not yet been referenced specifically in any publications.

Publishing research using ab7160? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Does this antibody recognize all IgG subtypes?

Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

Hello, I was wondering if any of your anti-human antibodies were tested against Fab' fragments. I wanted to make sure they would pick up heavy and light chain of a Fab' molecule. Thanks, Aparna

This antibody has been shown to react with both the heavy and light chains of human IgG.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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