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  1. Link

    rabbit-human-igg-hl-hrp-preadsorbed-ab7160.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Human IgG H&L (HRP) preadsorbed (ab7160)

  • Datasheet
Submit a review Q&A (2)

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit Anti-Human IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

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Overview

  • Product name

    Rabbit Anti-Human IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Human
  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity


    Mouse
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    human IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Enzyme
    • HRP

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
ELISA
1/72000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ICC/IF
1/1000 - 1/5000.
Notes
Dot blot
Use at an assay dependent concentration.
ELISA
1/72000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ICC/IF
1/1000 - 1/5000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab7160? Please let us know so that we can cite the reference in this datasheet.

ab7160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Does this antibody recognize all IgG subtypes?

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Abcam community

Verified customer

Asked on Apr 06 2005

Answer

Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

Read More

Abcam Scientific Support

Answered on Apr 15 2005

Question

Hello, I was wondering if any of your anti-human antibodies were tested against Fab' fragments. I wanted to make sure they would pick up heavy and light chain of a Fab' molecule. Thanks, Aparna

Read More

Abcam community

Verified customer

Asked on May 10 2002

Answer

This antibody has been shown to react with both the heavy and light chains of human IgG.

Read More

Abcam Scientific Support

Answered on May 13 2002

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