Rabbit Anti-Human IgG H&L (HRP) preadsorbed (ab7160)

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Key features and details

  • Rabbit Anti-Human IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

Overview

  • Product name

    Rabbit Anti-Human IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Human

  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details

  • Minimal
    cross-reactivity


    Mouse more details

  • Immunogen

    human IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/72000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
ICC/IF 1/1000 - 1/5000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab7160? Please let us know so that we can cite the reference in this datasheet.

ab7160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Does this antibody recognize all IgG subtypes?

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Answer

Thank you for your enquiry. These antibodies should recognize all human IgG subclasses. Please contact us again if you require further information.

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Question

Hello, I was wondering if any of your anti-human antibodies were tested against Fab' fragments. I wanted to make sure they would pick up heavy and light chain of a Fab' molecule. Thanks, Aparna

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Answer

This antibody has been shown to react with both the heavy and light chains of human IgG.

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