Product nameRabbit Anti-Human IgG H&L (Texas Red ®)
See all IgG secondary antibodies
Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ChIP, ELISAmore details
human IgG whole molecule
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesTexas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
ChIP Related Products
Our Abpromise guarantee covers the use of ab6757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at a concentration of 6 µg/ml (from PubMed: 17355974).
This product has been referenced in:
- Makino Y et al. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem 282:14073-82 (2007). Read more (PubMed: 17355974) »