Rabbit Anti-Human IgG H&L (TRITC) preadsorbed (ab7157)
- Datasheet
- References
- Protocols
Overview
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Product name
Rabbit Anti-Human IgG H&L (TRITC) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Mouse more details -
Immunogen
human IgG whole molecule
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Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
Our Abpromise guarantee covers the use of ab7157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IHC-P | ||
| IHC-Fr | ||
| Immunomicroscopy | ||
| Flow Cyt | ||
| ELISA |
Application notes
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
References
ab7157 has not yet been referenced specifically in any publications.
