Key features and details
- Rabbit Anti-Human IgG+IgM+IgA H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: ICC/IF
Product nameRabbit Anti-Human IgG+IgM+IgA H&L (FITC)
See all IgG + IgM + IgA secondary antibodies
SpecificityAntibodies were raised in rabbit to human IgG, IgM and IgA
Tested applicationsSuitable for: ICC/IFmore details
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound human immuno-globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immuno-globulins a minimum of 65% protein bound.
Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
General notesF/P ratio is 4-6 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where Ig's are depostited.
Our Abpromise guarantee covers the use of ab8512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8512 has been referenced in 1 publication.
- Pisa D et al. Evolution of antibody response and fungal antigens in the serum of a patient infected with Candida famata. J Med Microbiol 56:571-8 (2007). ICC/IF . PubMed: 17446276